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bovine serum albumin  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bovine serum albumin
    Bovine Serum Albumin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bsa+pbs/Blocker+BSA+(10X)+in+PBS/pmc13650043-207-0-4
    Average 94 stars, based on 1 article reviews
    bovine serum albumin - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: NOX4-derived oxidative DNA damage impairs thyroid differentiation through an epigenetic mechanism in BRAF-mutated radioactive iodine refractory papillary thyroid cancer cells
    Article Snippet: The cells were treated with 50μg/ml RNAse and incubated with anti-8-oxodG (Biotechne/R&D systems #4354-MC-050, 1:50 diluted in 2.5% BSA/PBS) or with mouse IgG2B isotype control (Biotechne/R&D systems #MAB004) for 1 h at 37 °C. .. The cells were then washed once with 0.2% BSA/PBS and incubated with the secondary antibodies Alexa 488 anti-mouse (Invitrogen, #A11017, RRID: AB_2534084) in 0.2% BSA/PBS for 1 h at 37 °C before propidium iodide staining. ..

    Article Title: The pro-inflammatory cytokines IFN-α and TNF-α inhibit organoid-derived extravillous trophoblast invasion
    Article Snippet: .. For whole-mount immunostaining in a 48-wells plate, non-specific binding sites of the tissue fragments were first blocked by incubation in 1% BSA/PBS with 1% Triton X-100 and 0.1% saponin (Acros Organics, 419231000) (blocking/permeabilization buffer) overnight at 4 °C. ..

    Article Title: Genomic and translational insights into eIF2B-mediated salt tolerance in sea rice HD961.
    Article Snippet: .. Then, cells were fixed in 4% (w/v) paraformaldehyde in PBS for 20 min at room temperature, washed three times in PBS, and permeabilized with 0.1% (v/v) Triton X-100 for 10 min. After blocking in 3% (w/v) BSA/PBS for 1 h, samples were incubated overnight at 4 °C with anti-eIF2B (sc-271332, Santa Cruz Biotechnology) diluted 1:200 in 1% BSA/PBS, washed three times in PBS, and then stained for 1 h at room temperature with goat anti-mouse Alexa Fluor 568 (Invitrogen) diluted 1:500 in 1% BSA/PBS. .. Protoplasts were mounted on poly-l-lysine–coated slides and imaged on a confocal laser-scanning microscope (Zeiss LSM series) using identical acquisition settings for FDA (Ex 488 nm/ Em 500–550 nm) and Alexa 568 (Ex 561 nm/Em 600– 630 nm).

    Article Title: Neutrophil-secreted extracellular vesicles induce blood-brain barrier leakage and tight junction disruption.
    Article Snippet: .. 259 Cells were incubated overnight at 4 °C with primary antibodies: claudin-5 260 (Mouse; 1:100; Thermo Fisher; #35-2500) and ZO-1 (Rabbit; 1:100; Thermo 261 Fisher; #40-22200) in 0.3% BSA/PBS with 0.2% Triton X-100. .. Slides were 262 washed and incubated with Alexa Fluor® 488-conjugated donkey anti-mouse 263 (or anti-rabbit) IgG (1:500; Invitrogen; #A21202 or #A21210) or Alexa 264 Fluor® 594-conjugated donkey anti-mouse (or anti-rabbit) IgG (1:500; 265 Invitrogen; #A21203 or #A21207) for 1 hour at room temperature, followed 266 by DAPI staining (Thermo Fisher; #62248) and mounted using ProLongTM 267 Diamond Antifade Mountant (Invitrogen; #P36961).

    Article Title: Downregulation of ankyrin 3 (ANK3) promotes malignant behaviors associated with altered adhesion dynamics and actin cytoskeleton remodeling in renal cell carcinoma.
    Article Snippet: Clear cell renal cell carcinoma (ccRCC) is the most common type of kidney cancer, with existing therapies largely ineffective for advanced stages.. Ankyrin 3 (ANK3), a protein involved in various cellular processes, has been associated with poor prognosis and immune infiltration in ccRCC.. However, its role in ccRCC development and progression remains poorly understood.

    Article Title: Divergent Regulation of Mammary Lipogenesis by trans -10, cis -12 and cis -9, trans -11 CLA Isomers Is Determined by Receptor-Specific Signaling
    Article Snippet: .. After treatment, the cells were fixed with 3% paraformaldehyde at room temperature for 20 min, washed three times with phosphate-buffered saline (PBS), and blocked in 2% bovine serum albumin (BSA) in PBS containing 0.2% Triton X-100 for 30 min. Coverslips were incubated in the dark with 2 μM BODIPY 493/503 (Invitrogen) prepared in 1% BSA/PBS with 0.1% Triton X-100 for 1 h. Nuclei were stained with Hoechst 33342 (Invitrogen), rinsed, and mounted on slides. .. Images were collected via a Nikon Eclipse Ts2R microscope (Nikon, Tokyo, Japan) with 405 nm or 488 nm excitation lines and analyzed via FIJI (ImageJ; https://imagej.net/ij/ , accessed on 15 May 2024).

    Staining:

    Article Title: NOX4-derived oxidative DNA damage impairs thyroid differentiation through an epigenetic mechanism in BRAF-mutated radioactive iodine refractory papillary thyroid cancer cells
    Article Snippet: The cells were treated with 50μg/ml RNAse and incubated with anti-8-oxodG (Biotechne/R&D systems #4354-MC-050, 1:50 diluted in 2.5% BSA/PBS) or with mouse IgG2B isotype control (Biotechne/R&D systems #MAB004) for 1 h at 37 °C. .. The cells were then washed once with 0.2% BSA/PBS and incubated with the secondary antibodies Alexa 488 anti-mouse (Invitrogen, #A11017, RRID: AB_2534084) in 0.2% BSA/PBS for 1 h at 37 °C before propidium iodide staining. ..

    Article Title: Genomic and translational insights into eIF2B-mediated salt tolerance in sea rice HD961.
    Article Snippet: .. Then, cells were fixed in 4% (w/v) paraformaldehyde in PBS for 20 min at room temperature, washed three times in PBS, and permeabilized with 0.1% (v/v) Triton X-100 for 10 min. After blocking in 3% (w/v) BSA/PBS for 1 h, samples were incubated overnight at 4 °C with anti-eIF2B (sc-271332, Santa Cruz Biotechnology) diluted 1:200 in 1% BSA/PBS, washed three times in PBS, and then stained for 1 h at room temperature with goat anti-mouse Alexa Fluor 568 (Invitrogen) diluted 1:500 in 1% BSA/PBS. .. Protoplasts were mounted on poly-l-lysine–coated slides and imaged on a confocal laser-scanning microscope (Zeiss LSM series) using identical acquisition settings for FDA (Ex 488 nm/ Em 500–550 nm) and Alexa 568 (Ex 561 nm/Em 600– 630 nm).

    Article Title: Divergent Regulation of Mammary Lipogenesis by trans -10, cis -12 and cis -9, trans -11 CLA Isomers Is Determined by Receptor-Specific Signaling
    Article Snippet: .. After treatment, the cells were fixed with 3% paraformaldehyde at room temperature for 20 min, washed three times with phosphate-buffered saline (PBS), and blocked in 2% bovine serum albumin (BSA) in PBS containing 0.2% Triton X-100 for 30 min. Coverslips were incubated in the dark with 2 μM BODIPY 493/503 (Invitrogen) prepared in 1% BSA/PBS with 0.1% Triton X-100 for 1 h. Nuclei were stained with Hoechst 33342 (Invitrogen), rinsed, and mounted on slides. .. Images were collected via a Nikon Eclipse Ts2R microscope (Nikon, Tokyo, Japan) with 405 nm or 488 nm excitation lines and analyzed via FIJI (ImageJ; https://imagej.net/ij/ , accessed on 15 May 2024).

    Immunostaining:

    Article Title: The pro-inflammatory cytokines IFN-α and TNF-α inhibit organoid-derived extravillous trophoblast invasion
    Article Snippet: .. For whole-mount immunostaining in a 48-wells plate, non-specific binding sites of the tissue fragments were first blocked by incubation in 1% BSA/PBS with 1% Triton X-100 and 0.1% saponin (Acros Organics, 419231000) (blocking/permeabilization buffer) overnight at 4 °C. ..

    Binding Assay:

    Article Title: The pro-inflammatory cytokines IFN-α and TNF-α inhibit organoid-derived extravillous trophoblast invasion
    Article Snippet: .. For whole-mount immunostaining in a 48-wells plate, non-specific binding sites of the tissue fragments were first blocked by incubation in 1% BSA/PBS with 1% Triton X-100 and 0.1% saponin (Acros Organics, 419231000) (blocking/permeabilization buffer) overnight at 4 °C. ..

    Blocking Assay:

    Article Title: The pro-inflammatory cytokines IFN-α and TNF-α inhibit organoid-derived extravillous trophoblast invasion
    Article Snippet: .. For whole-mount immunostaining in a 48-wells plate, non-specific binding sites of the tissue fragments were first blocked by incubation in 1% BSA/PBS with 1% Triton X-100 and 0.1% saponin (Acros Organics, 419231000) (blocking/permeabilization buffer) overnight at 4 °C. ..

    Article Title: Genomic and translational insights into eIF2B-mediated salt tolerance in sea rice HD961.
    Article Snippet: .. Then, cells were fixed in 4% (w/v) paraformaldehyde in PBS for 20 min at room temperature, washed three times in PBS, and permeabilized with 0.1% (v/v) Triton X-100 for 10 min. After blocking in 3% (w/v) BSA/PBS for 1 h, samples were incubated overnight at 4 °C with anti-eIF2B (sc-271332, Santa Cruz Biotechnology) diluted 1:200 in 1% BSA/PBS, washed three times in PBS, and then stained for 1 h at room temperature with goat anti-mouse Alexa Fluor 568 (Invitrogen) diluted 1:500 in 1% BSA/PBS. .. Protoplasts were mounted on poly-l-lysine–coated slides and imaged on a confocal laser-scanning microscope (Zeiss LSM series) using identical acquisition settings for FDA (Ex 488 nm/ Em 500–550 nm) and Alexa 568 (Ex 561 nm/Em 600– 630 nm).

    Saline:

    Article Title: Divergent Regulation of Mammary Lipogenesis by trans -10, cis -12 and cis -9, trans -11 CLA Isomers Is Determined by Receptor-Specific Signaling
    Article Snippet: .. After treatment, the cells were fixed with 3% paraformaldehyde at room temperature for 20 min, washed three times with phosphate-buffered saline (PBS), and blocked in 2% bovine serum albumin (BSA) in PBS containing 0.2% Triton X-100 for 30 min. Coverslips were incubated in the dark with 2 μM BODIPY 493/503 (Invitrogen) prepared in 1% BSA/PBS with 0.1% Triton X-100 for 1 h. Nuclei were stained with Hoechst 33342 (Invitrogen), rinsed, and mounted on slides. .. Images were collected via a Nikon Eclipse Ts2R microscope (Nikon, Tokyo, Japan) with 405 nm or 488 nm excitation lines and analyzed via FIJI (ImageJ; https://imagej.net/ij/ , accessed on 15 May 2024).

    Cell Characterization:

    Article Title: Sample-multiplexed FACS-preprocessing of PBMCs enables scalable scRNA-seq without compromising transcriptomic or cellular integrity
    Article Snippet: .. The PBMC pellet was resuspended in 100 μL of 0.1% BSA/PBS, and a second cell count and viability assessment was performed using a Countess II Automated Cell Counter (Invitrogen). ..



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